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Image Search Results
Journal: Bioengineered
Article Title: Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p
doi: 10.1080/21655979.2022.2053814
Figure Lengend Snippet: Effect of TTTY15 on GC cell proliferation, apoptosis, migration, invasion and EMT process. (a) The transfection efficiency of TTTY15 overexpression plasmid, si-TTTY15-1 and si-TTTY15-2 was detected by qRT-PCR. (b and c) CCK-8 method and BrdU assay were conducted for detecting the effects of TTTY15 overexpression and knockdown on the viability and proliferation of NCI-N87 and SUN-1 cells. (d) Flow cytometry assay was utilized for detecting the effects of TTTY15 overexpression and knockdown on the apoptosis of NCI-N87 and SUN-1 cells. (e) Transwell assays were performed to detect the effects of TTTY15 overexpression and knockdown on the migration and invasion of NCI-N87 and SUN-1 cells. (f) Western blot was carried out to detect the effects of TTTY15 overexpression and knockdown on the expression of EMT-related proteins in NCI-N87 and SUN-1 cells. All of the experiments were performed in triplicate. * P < 0.05, ** P < 0.01 and *** P < 0.001.
Article Snippet: The
Techniques: Migration, Transfection, Over Expression, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, BrdU Staining, Flow Cytometry, Western Blot, Expressing
Journal: Bioengineered
Article Title: Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p
doi: 10.1080/21655979.2022.2053814
Figure Lengend Snippet: Effects of TTTY15/let-7a-5p axis on GC cell proliferation, apoptosis, migration and invasion. (a) qRT-PCR was used to detect the transfection efficiency of TTTY15 overexpression plasmids + miR mimics and si-TTTY15-1 + miR inhibitors. (b and c) CCK-8 method and BrdU assay were used to detect the effects of TTTY15 and let-7a-5p on the viability and proliferation of NCI-N87 and SUN-1cells. (d) Flow cytometry assay was used to detect the effects of TTTY15 and let-7a-5p on NCI-N87 and SUN-1 cell apoptosis. (e and f) Transwell assays were used to detect the effects of TTTY15 and let-7a-5p on the migration and invasion of NCI-N87 and SUN-1 cells. (g) Western blot was used to detect the effects of TTTY15 and let-7a-5p on the EMT process of NCI-N87 and SUN-1 cells. All of the experiments were performed in triplicate. ** P < 0.01 and *** P < 0.001.
Article Snippet: The
Techniques: Migration, Quantitative RT-PCR, Transfection, Over Expression, CCK-8 Assay, BrdU Staining, Flow Cytometry, Western Blot